Figure 1: Protegrin-1 deep mutational SLAY predicts residue importance and flexibility.

A) Surface localized antimicrobial display expresses an OmpA fusion protein tethering the PG-1 library to the outer membrane (OM). Induction of display results in cell death for variants maintaining antimicrobial activity (red-active, blue-inactive). change in reads between induced and uninduced cultures can predict antimicrobial potential. B) Scatter plot of induced versus uninduced reads for the entire PG-1 library on log scales. 1:1 and the native PG-1 sequence (PG-1.0) read ratios are shown. C) Scatter plot of MIC for a subset of 52 variants in the PG-1 library versus the log₂-fold change in reads. D) Table charting dmSLAY predictions for all single PG-1 variants in the library. The native PG-1 sequence and position in columns one and two, amino acid change at each position going across the top categorized by side chain. PG-1 secondary structure and disulfide bonds are diagramed to the left. Position column is color coded by average log₂-fold change. Bold boxed cells were evaluated in vitro. An X marks an incorrect dmSLAY prediction.