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[Preprint]. 2023 Jul 29:2023.07.27.550871. [Version 1] doi: 10.1101/2023.07.27.550871

Figure 3. TGF-β inhibits cytotoxicity from recently reactivated memory CD8+ T cells and short-term exposure to TGF-β inhibits cytotoxicity of subsequently activated memory CD8+ T cells.

Figure 3.

(A) Schematic of ex vivo stimulation; cells were stimulated for 24 hours with CD3/28, N4, Q4, Cyt, and TGF-β at 100ng/mL. TGF-β was added 0 hours, 6 hours, or 12 hours post-start of activating stimulation. (B) Frequencies of IFN-γ and (C) GzmB in OT-I Tmem compared across stimulation conditions with TGF-β addition at indicated timepoints (n = 8 animals). (D) Schematic of ex vivo stimulation of isolated T cells from OT-I memory mice. Cells were treated with 100ng/mL TGF-β or media alone for 2 hours, the TGF-β was then washed out (down to 0.001ng/mL), immediately followed by 24 hours of activating stimulation. Stimulations were CD3/28, N4, Q4, Cyt, and TGF-β at 100ng/mL. (E) Frequencies of IFN-γ and (F) GzmB in OT-I T cells compared across stimulation conditions. CD3/28 data depicted are from n = 7 animals, N4 from n = 13, Q4 from n = 4, and Cyt from n = 9. All indicated statistical significances were calculated using one-way ANOVA with Tukey’s multiple comparison test. Data shown are from 3 to 10 independent experiments.