Extended Data Fig. 8. DNA unwrapping can occur with both entry and exit side DNA.
a) Coulomb potential maps from refinements of subsets of particles corresponding to endpoints of principal components 3 and 4 from 3D variability analysis of the single-bound SNF2h-nucleosome particles. For these two components, the entry side DNA unwraps, leading to loss of H3 and H2A densities for component 3 but not for component 4.
b) Coulomb potential maps from refinements of subsets of particles corresponding to endpoints of principal component 5 from 3D variability analysis of the single-bound SNF2h-nucleosome particles. In this component, the exit side DNA unwraps, leading to loss of adjacent H3 and H2A densities.