Extended Data Fig. 1. ssDNA GO grids protect nucleosomes from the AWI.

a) Schematic illustrating the use of complementary DNA sequences to bring nucleosomes closer to the GO surface away from the AWI.

b) Representative micrograph of nucleosomes with complementary ssDNA overhang on an ssDNA GO grid.

c) Representative micrographs of nucleosomes on an ssDNA GO grid. Areas without GO display denatured nucleosomes (upper left). A hole partially covered with GO shows nucleosomes are only visible in the area with GO (upper right). The nucleosomes appear uniformly spread across holes covered with GO (lower left and lower right).

d) Representative 2D classes showing intact nucleosomes on an ssDNA GO grid.