Table 1.
Antibody | Company | Catalogue Number |
Clone | Concentration for Immunofluorescence | Concentration for Western Blot |
---|---|---|---|---|---|
GFAP(AF488) | Invitrogen | 53–9892-82 | GA5 | 10 μg/mL | |
GLUT-1 | Invitrogen | PA5–16793 | Poly | 1:100 | |
CD71 | Invitrogen | 14–0719-82 | OKT9 | 5 μg/mL | |
PECAM(FITC) | Invitrogen | 11–0311-82 | 390 | 5 μg/mL | |
VE-cadherin | Invitrogen | 14–1449-82 | 16B1 | 5 μg/mL | |
Zo-1 | Invitrogen | 61–7300 | Poly | 10 μg/mL | 1 μg/mL |
Occludin | Invitrogen | 71–1500 | Poly | 4 μg/mL | 0.5 μg/mL |
Claudin 5 | Invitrogen | 35–2500 | 4C3C2 | 1:25 | 1:1000 |
OAT1 | Invitrogen | PA5–26244 | Poly | 1:100 | 1:1000 |
OAT3 | Invitrogen | PA5–76143 | Poly | 1:100 | 1:1000 |
ENT1 | P rote intech | 11337–1-AP | Poly | 1:100 | |
ENT1 | Invitrogen | PA5-116461 | Poly | 1:500 | |
OATP1A2 | Invitrogen | PA5–42445 | Poly | 1:100 | 1:1000 |
OATP2A1 | Invitrogen | PA5–98789 | Poly | 1:200 | 1:1000 |
BCRP | Novus | NBP2-22124 | 3G8 | 1:200 | 1:2000 |
P-gp | Invitrogen | PA5–61300 | Poly | 2 μg/mL | |
P-gp | Abcam | ab261736 | Poly | 0.5 μg/mL | |
MRP1 | Abcam | ab24102 | MRPm5 | 1:50 | 1:200 |
MRP4 | Cell Signaling |
12705 | D2Q2O | 1:200 | 1:1000 |
MRP5 | Invitrogen | PA5–18965 | Poly | 10 μg/mL | |
MRP5 | Abcam | Ab180724 | Poly | 1:1000 | |
CYP3A4 | Invitrogen | MA5–17064 | 3H8 | 1:200 | 1:1000 |
PXR | Invitrogen | PA5–72551 | Poly | 1:50 | 1:1000 |
CAR | R&D Systems |
PP-N4111-00 | N4111 | 10 μg/mL | 2 μg/mL |
AK1 | Invitrogen | PA5–52297 | Poly | 1 μg/mL | 0.1 μg/mL |
AK2 | Proteintech | 11014–1-AP | Poly | 1:200 | 1:1000 |
AK5 | Invitrogen | PA5–53933 | Poly | 1.2 μg/mL | 0.09 μg/mL |
AK6 | Novus | NBP2-67116 | B1-F4 | 1:100 | 1:2000 |
B-Actin (HRP) | Cell Signaling |
12262 | 8H10D 10 |
1:5000 | |
Anti-rabbit (HRP) |
Abcam | ab97051 | Poly | 1:2000 | |
Anti-mouse (HRP) |
Abcam | ab97023 | Poly | 1:2000 | |
Anti-rabbit (AF488) |
Invitrogen | A-11008 | Poly | 1 μg/mL | |
Anti-mouse (AF488) |
Invitrogen | A-11001 | Poly | 0.67 μg/mL | |
Anti-goat (AF488) |
Invitrogen | A-11078 | Poly | 1 μg/mL |