Table 2.
Protein copy number and concentration following 24-hour treatment with cocaine (10 μM) or vehicle as determined by proteomics analysis. Mean ± standard deviation are shown. Asterisks indicate statistical significance in cocaine treated cells relative to vehicle.
| Vehicle | Cocaine | |||
|---|---|---|---|---|
| Protein | Copy Number | Concentration (nM) | Copy Number | Concentration (nM) |
| CEACAM1 | 59,626 ±10,789 | 43.9 ± 7.5 | 106,220 ± 7,458** | 86.6 ± 4.4** |
| COL6A1 | 206,854 ± 12,118 | 152.2 ± 3.7 | 131,021 ± 14,120** | 106.9 ± 11.7** |
| CTSB | 8,484,178 ± 416,822 | 6,252.0 ± 355.4 | 6,593,383 ± 408,852** | 5,377.0 ± 230.0* |
| HPX | 6,543,012 ± 138,928 | 4,821.0 ± 139.0 | 4,814,035 ± 116364**** | 3,931.0 ± 184.8** |
| ICAM | 676,996 ± 14,380 | 499.0 ± 24.0 | 721,746 ± 23,606* | 588.8 ± 7.0** |
| MIF | 3,493,966 ± 119,612 | 2,573.0 ± 39.4 | 4,902,856 ± 535,590* | 3,996.0 ± 358.4** |
| PECAM | 1,202,038 ± 59,972 | 885.6 ± 46.24 | 956,743 ± 10,063** | 780.8 ± 9.6* |
| PICALM | 1,014,023 ± 21,188 | 747.1 ± 20.5 | 788,182 ± 30,189*** | 643.2 ± 25.7** |
| NRP2 | 287,851 ± 21,441 | 212.0 ± 13.9 | 186,651 ± 2,301** | 152.3 ± 3.6** |
| VTN | 1,208,330 ± 68,554 | 890.8 ± 65.2 | 862,826 ± 6,116*** | 704.3 ± 20.8** |
| VWF | 584,018 ± 20,536 | 430.1 ± 9.7 | 484,891 ± 17,129** | 395.6 ± 11.0* |
*
p<0.05.
**
p<0.01.
***
p<0.001.
****
p<0.0001. Paired T-test. COL6A1, Collagen Type VI Alpha 1 Chain. CTSB, Cathepsin B. HPX, Hemopexin. MIF, Macrophage Migration Inhibitory Factor. PICALM, Phosphatidylinositol Binding Clathrin Assembly Protein. NRP2, Neuropilin 2. VTN, Vitronectin. VWF, Von Willebrand Factor.