FIG. 2.

Solubilization of Vif and Gag. HIV-1-infected H9 cells were lysed in PBS–1% TX-100, and the nuclei were pelleted by low-speed centrifugation. Various detergents were added to aliquots of the postnuclear supernatant to a final concentration of 1%, or salt was added to a final concentration of 1 M. These were then centrifuged at 100,000 × g to separate soluble (S) from insoluble (I) fractions. Aliquots of the fractions corresponding to equal numbers of cells were examined by Western blot analysis using antibodies specific for calreticulin, HIV-1 CA, or HIV-1 Vif.