FIG. 5.
Quantitation of Vif and Gag recovered by high-speed centrifugation and immunoprecipitation. The fraction 4 (Fig. 4) samples that had been either pelleted, or immunoprecipitated (IP) with anti-CA or preimmune (pre-imm.) serum were resolved on SDS-polyacrylamide gels together with dilution series of purified recombinant His6-tagged Vif and CA (which served as standard curves). The proteins were visualized by using monoclonal antibodies, chemiluminescence, and autoradiography, and the bands were quantitated by densitometry. Short (lanes 1 to 8) and long (lanes 9 and 10) exposures of the final filters are shown. Note that three times as much sample was loaded on this gel as for the gels shown in Fig. 4.