TABLE 1.

Quantitation of Vif and Gag

Sample	Vifa (ng/sample)	Gagb (ng/sample)	No. of moleculesc (1014)		Vif/Gag ratiod
			Vif	Gag
Pelleted	5	11	19	36	0.5
mImunoprecipitate; anti-CA	<0.032	1.5	<0.1	5	∼0
Immunoprecipitate; preimmune	<0.032	<0.032	<0.1	<0.1	ND

^aTotal amount of protein reactive with the Vif-specific monoclonal antibody, determined for each sample by using the dilution series of recombinant protein as the standard curve (Fig. 5), the calculated relative molecular masses of the native and recombinant proteins, and densitometry. 

^bThe total amount of protein (including p24^Gag, p55^Gag, and the various processing intermediates) reactive with the CA-specific monoclonal antibody, determined for each sample by using the dilution series of recombinant protein as the standard curve (Fig. 5), the calculated relative molecular masses of the native and recombinant proteins, and densitometry. 

^cCalculated by using molecular masses, amounts of each protein, and Avogadro’s constant. 

^dMolar ratio of Gag/Vif calculated as described in footnote c.