Fig. 3. Dsup promotes survival after oxidative DNA damage in a manner that requires its chromatin binding C-terminus but is not due to ROS scavenging.

(a). Five-fold serial dilution analysis of yeast expressing Dsup alleles plated on H₂O₂ (concentrations as indicated). (b). Cell survival after 90-minute exposure to indicated concentrations of H₂O₂. Shown are average and standard deviation of experiments performed from three independent yeast colonies for each strain. * = p < 0.05, ** = p < 0.01, *** = p < 0.001 by student’s t-test. (c). Nuclear ROS (measured by Redox analysis as in Methods) for indicated yeast strains. Shown are average and standard deviation of experiments performed from three independent colonies. No significant differences were observed between each time point.