Skip to main content
[Preprint]. 2023 Jul 26:rs.3.rs-3147210. [Version 1] doi: 10.21203/rs.3.rs-3147210/v1

Figure 2. Detection of NICD homo- and heterodimer complexes.

Figure 2

293T cells were transfected with FLAG- and HA-tagged species of various NICDs and dimerization was detected by co-immunoprecipitation with anti-FLAG antibodies and western blotting with anti-HA antibodies. In all panels, Input represents 10% of whole cell lysates before Co-IP. The heterodimerization possibilities are represented with (A) N1ICD, (B) N2ICD, (C) N3ICD, and (D) N4ICD. To prevent confounding results from residual unstripped antibodies, lysates were run twice on two different membranes for cleaner western blot analysis. Panel D also includes an example of a typical negative FLAG-tagged NICD lane (−), controlling for non-specific interactions between HA-tagged protein on anti-FLAG antibody resins.