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. 2023 Jul 20;14:1201064. doi: 10.3389/fmicb.2023.1201064

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Copyright © 2023 Petrone, Rios Glusberger, George, Milletich, Ahrens, Roesch and Triplett.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alpha- and beta-diversity measures of the R10.4 rrn Illumina comparison. (A) Alpha diversity by the measure of Simpson Evenness and (B) Observed richness. The database is faceted on top of each read grouping by database while the sequence type can be seen as red (Illumina), green (Pseudo-V3V4), and blue (RRN). Kruskal–Wallis and pairwise chi-squared were done and p.adj is shown. (C) Beta-dispersion metrics of distance to the centroid. Sprawl between sample centers can be seen on the y-axis while each sequence type is plotted separately on the x-axis. Non-parametric ANOVA and pairwise t-test are shown here. (D) Beta-dispersion PCoA by Bray–Curtis distance matrix. Each sequence type can be seen as a distinct shape or by color: Illumina (black), Pseudo-V3V4 (red), and RRN (green).