Table 3.

ONT R10.4 output classification of the 4.5 kb rrn reads within the ZymoBIOMICS microbial DNA standard.

Genus	Species	NCBI RRN	EMU	RDP	Silva-138	Theoretical
	Uncultured soil bacterium			0.98
Bacillus	Bacillus halotolerans		0.29
Bacillus	Bacillus intestinalis			0.30
Bacillus	Bacillus spizizenii			16.92
Bacillus	Bacillus subtilis	25.89	24.88	6.02		17.40
Bacillus	Uncultured Bacillus sp.			0.13
Enterococcus	Enterococcus faecalis	4.10	4.50	4.47		9.90
Escherichia	Escherichia coli	3.33	3.34	3.23		10.10
Klebsiella	Klebsiella pneumoniae				6.17
Limosilactobacillus	Limosilactobacillus fermentum	17.25	16.54	16.97		18.40
Listeria	Listeria monocytogenes	14.81	13.85	13.43		14.10
Pseudomonas	Pseudomonas aeruginosa	1.35	1.20	1.22		4.20
Salmonella	Salmonella enterica	1.47	1.41	1.44		10.40
Staphylococcus	Staphylococcus aureus	30.95	33.33	32.67		15.50
Staphylococcus	uncultured Staphylococcus sp.			0.13
Streptococcus	Streptococcus pneumoniae				93.11
Streptococcus	uncultured Streptococcus sp.			0.14

Relative abundances are shown as the average across four replicates after applying sample abundance threshold filtering. Four databases were tested: the NCBI RRN database (NCBI RRN), the Ribosomal Database Project (RDP), the EMU database (EMU), and the Silva-138 database (Silva-138). The values for the relative abundance of the Mock were obtained through the supplier's website and attached documents. Taxa that were not found using specific databases are presented as missing values.