TABLE 1.

Site-directed mutagenesis of the HIV-1 Vif protein^a

Name	Position	Mutation	Relative infectivity	SD
M1	5–6	WQ→AA	56	11
M2	12–13	QV→AA	115	17
M38	16–18	MRI→AAA	71	16
M3	22–23	RL→AA	86	10
M20	29–31	MYI→AAV	41	14
M4	33–34	RK→AA	65	18
M21	38–40	WFY→AAA	6	5
M5	43–44	HY→AA	5	1
M22	53–54	SE→AA	61	22
M6	58–59	PL→AA	55	18
M23	69–70	YW→AA	3	3
M7	73–74	HT→AA	116	23
M24	80–81	HL→AA	81	8
M8	86–87	SI→AA	58	7
M25	90–92	RKK→AAA	118	55
M9	97–98	QV→AA	73	6
M26	105–107	QLI→AAV	2	1
M10	111–112	YF→AA	7	7
M18	114	C→S	2	1
M27	121–123	RNT→AAA	135	7
M11	127–128	RI→AA	90	21
M19	133	C→S	5	1
M28	135–136	YQ→AA	27	4
M12	140–141	NK→AA	32	6
M29	144–146	SLQ→AAA	7	6
M13	147–148	YL→AA	60	4
M39	156–158	PKQ→AAA	103	51
M30	157	K→A	114	23
M40	158–160	QIK→AAA	91	17
M31	160	K→A	67	7
M32	161	P→A	73	16
M33	162	P→A	99	36
M34	163	L→A	74	4
M14	161–163	PPL→AAA	3	1
M35	164	P→A	37	20
M41	161–164	PPLP→APLA	12	13
M36	165	S→A	33	6
M37	166	V→A	80	35
M15	169–170	LT→AA	58	6
M16	180–181	TK→AA	95	11
M17	189–190	MW→AA	96	10
HIV-1/Δvif	26–192	NA	1
HIV-1	NA	NA	100

^aThe positions, identities, and nomenclature of the amino acid substitutions that were introduced into the Vif protein of HIV-1_HXB3 are listed together with their relative abilities to confer infectivity on a vif-deficient HIV-1. Vif function was determined by cotransfection of H9 cells with pIIIB/Δvif, virus harvest and normalization, and single-cycle infectivity assays using C8166/HIV-CAT indicator cells. The mean infectivity values (and their standard deviations) are set against values of 100 for wild-type Vif and 1 for the negative control (Δvif) following correction for background CAT activity (mock infection). In most cases, the data reflect at least three independent experiments; the exceptions are M24, M27, M39, M30, M41, M36, M16, and M17, which were each tested twice. NA, not applicable.