Table 5.
Mediation analysisa for the top CpGs from DMRs significantly associated with 3rd trimester O3, and with consistent direction of association with the outcome of adiposity at 5 months of age among 294 infants.
| CpG | Annotated gene(s) | Direction of association with 3rd trimester O3 | Direction of association with 5-month adiposity | Indirect effect (95% CI)b | Direct effect (95% CI)b | Proportion mediated (95% CI)b |
|---|---|---|---|---|---|---|
| cg03344955 | MCCC1 | negative | negative | 19.95 (−43.94, 89.47) | 197.32 (−28.09, 421.17) | 0.08 (−0.46, 0.89) |
| cg19626725 | RUFY1 | negative | negative | −4.2 (−47.76, 37.70) | 223.38 (4.50, 437.28) | −0.01 (−0.43, 0.35) |
| cg07372500 | LOC100128340 | negative | negative | −1.90 (−48.47, 45.10) | 221.39 (−0.61, 438.99) | −0.01 (−0.45, 0.38) |
| cg25181043 | LINC00494 | negative | negative | 6.31 (−34.21, 49.29) | 210.86 (−10.89, 427.14) | 0.02 (−0.33, 0.45) |
| cg07396746 | LOXL2 | negative | negative | 8.31 (−11.90, 40.50) | 210.72 (−3.35, 427.21) | 0.02 (−0.10, 0.32) |
| cg11678027 | CCDC169; CCDC169-SOHLH2 | negative | negative | 4.43 (−22.90, 36.45) | 214.81 (1.35, 428.05) | 0.01 (−0.21, 0.31) |
a
Conducted using the R package mediation, as described in Tingley et al. (2014).
b
Estimate and 95% quasi-Bayesian confidence interval, from models adjusted for average temperature during the exposure period of interest, infant sex, maternal education, maternal race/ethnicity, season of birth, infant gestational age at birth, technical batch, and estimated proportions of seven cell types (B cells, CD4+ T cells, CD8+ T cells, granulocytes, monocytes, NK cells, and nucleated red blood cells).