FIG. 3.
EMSA of wild-type Rep78 synthesized in vitro. Each lane contained 20,000 cpm of 32P-labeled RBS oligonucleotide probe. Lane 1 contained unprogrammed lysate. When lysate programmed with pCMVR78 was incubated with the probe, multiple shifted bands (bracket) were observed (lane 2). Shifted bands disappeared gradually as the amount of cold competitor added increased (lanes 3 and 4). The higher-mobility band (arrowhead) is completely depleted when anti-Rep antibody 76.3 is included in the reaction, whereas other slower bands (∗) are not likely to be supershifted (compare lane 5 to lane 2).