TABLE 2.
Coat protein accumulations of the BaMV infectious clone (pBaMV-O) and its derivatives in protoplasts of N. benthamiana
Clone | Mutation(s) | Coat protein accumulationa |
---|---|---|
pBaMV-O (wild type) | 1.00 | |
pBaMV-O/G1 | C1 → G1 | 0.15 ± 0.05 |
pBaMV-O/C19 | G19 → C19 | 0.13 ± 0.06 |
pBaMV-O/G1C19 | C1 → G1/G19 → C19 | 0.75 ± 0.12 |
pBaMV-O/C18 | G18 → C18 | 0.12 ± 0.06 |
pBaMV-O/G2C18 | C2 → G2/G18 → C18 | 0.56 ± 0.09 |
pBaMV-O/C18C19 | G18 → C18/G19 → C19 | 0.17 ± 0.04 |
Coat protein levels determined by Western blotting compared to that of pBaMV-O; data were taken from an average of at least four independent runs of protoplast inoculation, and each inoculation was subjected at least two times to Western blot analysis.