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. Author manuscript; available in PMC: 2023 Aug 4.
Published in final edited form as: Gut. 2023 Mar 8;72(6):1174–1185. doi: 10.1136/gutjnl-2022-328154

Fig. 3. iHUBs mediate chemoresistance in PDAC.

Fig. 3

(a,b) Occupancy profiles of H3K27ac, H3K4me3, ATAC at LIF and GPX4 iHUBs in Par and PacR cells showing minimal changes in H3K27ac and H3K4me3, but significant gain of BRD4 occupancy [RPKM]. Arcs in the HiChIP data show an increase in interaction frequency in PacR (pink) compared to Par cells (gray). (c) Quantitative PCR for LIF and GPX4 mRNAs in control cells (MOCK) or following deletion of LIF or GPX4 iHUBs. Both LIF and GPX4 mRNAs were significantly downregulated upon the deletion of their respective iHUBs in PacR. Deletion of iHUBs in Par did not affect LIF and GPX4 mRNA levels, supporting a specific role of iHUBs in resistance. (d Crystal violet staining for proliferation assay upon deletion of LIF or GPX4 iHUBs compared to MOCK showing sensitization of PacR cells when they lose one iHUB.