Skip to main content
. 2023 Jun 2;10(8):uhad119. doi: 10.1093/hr/uhad119

Figure 3.

Figure 3

GA3 sensitivity of the BraRGL1 mutants. a Subcellular localization of BraRGL1 in Nicotiana benthamiana. DsRed was used to stain the nuclei. Scale bar = 50 μm. b Detection of interactions between BraRGL1 proteins and BraGID1b after treatment with 100 mg/L GA3. BraRGL1-M represents the mutated protein. AD and BD represent empty pGADT7 and pGBKT7, respectively. SD/−Trp-Leu means medium lacked tryptophan and leucine; SD/−Trp-His-Leu-Ade means medium lacked tryptophan, histidine, leucine, and adenine. c Sensitivity of BraRGL1 mutants to GA3 determined using a hypocotyl elongation assay with 100 mg/L GA3 treatment. Scale bar = 1 cm. d Quantification of hypocotyl lengths, as in Figure 2. n, number of hypocotyls.