TABLE 1.
Nucleotide and amino acid sequence differences in the Bst11107I-BssHII region between vSVLSJ1 and vSVLS00
| Nucleotide no. | vSVLSJ1a
|
vSVLS00a
|
Amino acid no. | Mutation siteb | ||
|---|---|---|---|---|---|---|
| nt | aa | nt | aa | |||
| 1C, 2374 | T | Val | C | Ala | 544 | 301M |
| 1D | ||||||
| 2466 | A | Met | G | Val | 575 | 101M |
| 2500 | C | Thr | T | Ile | 586 | 102M |
| 2832 | T | Ser | C | Pro | 697 | 103M |
| 2842 | A | Gln | G | Arg | 700 | 104M |
| 3073 | T | Ile | C | Thr | 777 | 105M |
| 3243 | A | Lys | G | Glu | 834 | 106M |
| 2A, 3355 | G | Arg | T | Ile | 871 | 201M |
a
nt, nucleotide; aa, amino acid.
b
Each nucleotide was termed as shown in the table for site-directed mutagenesis.