FIG. 5.

Incorporation of gE proteins into virions. PK15 cells were infected at an MOI of 10 with either PRV Be (wild type), PRV 107 (Am457), PRV 25 (frameshift), PRV 104 (Y478S), PRV 105 (Y478S + Y517S), or PRV 106 (Y517S) for 14 h. Medium was removed from the cells, and released virions were isolated by centrifugation through 30% sucrose. Western blot analysis was performed with a rabbit polyclonal antiserum to gE. The same total volume of extract was loaded in each lane except for lane 3, in which twice as much total protein was analyzed. Positions of apparent molecular mass markers are indicated in kilodaltons on the left.