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Relative sensitivity of the established triple-pathogen RT-qPCR specific for swine influenza A virus, porcine respirovirus 1 and swine orthopneumovirus, respectively.
| RNA dilution | 3plex RT-qPCR (Cq-values) | ||
|---|---|---|---|
| PRV1 | SOV | M | |
| 0 | 23.56 | 22.86 | 23.29 |
| −1 | 27.62 | 26.10 | 26.36 |
| −2 | 31.00 | 29.31 | 29.63 |
| −3 | 34.16 | 32.68 | 32.61 |
| −4 | 37.58 | 35.27 | 36.25 |
| −5 | neg | neg | Neg |
| −6 | neg | neg | Neg |
