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. 2023 Aug 3;38(1):2242601. doi: 10.1080/14756366.2023.2242601

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 6. — Comparison of the antiproliferative ability of compound 19f, BAY-850 and AM879 on BT-549 cells. (A) BT-549 cells were treated with 10 μM compound 19f, BAY-850 and AM879, the newly synthesised DNA was detected using EdU-488, and the nuclei were labelled with Hoechst 33258. Scale bar = 200 μm. (B) Relative EdU-488 intensity was quantified with Image J, and the mean intensity value of the control group was 100%. ****p < 0.0001, compared with the control group. (C) Colony formation assay were used to detect the long-term cell proliferation ability after compound 19f, BAY-850 and AM879 treatment. Relative colony formation ability was quantified with Image J, and the mean value of the control group was 100%. ****p < 0.0001, compared with the control group. (D) 3D spherical plates were used to investigate the effect of compounds on the formation of cell spheres. Relative 3D cell spheres diameter was quantified with Image J, and the mean value of the control group was 100%. ****p < 0.0001, compared with the control group.