Figure 2.

Musashi-target lineage restricted mRNAs. (A) Musashi target mRNAs with pituitary cell lineage-restricted expression. Prior pituitary single cell RNA sequencing has reported mRNAs showing cell lineage-restricted expression in the mouse or rat (54, 78, 79). Here we cross reference those mRNAs with mRNAs enriched >2-fold in Musashi IPs (Supplementary Table S2 (76)). (B) Musashi exerts repression via the Fshb 3′ UTR. NIH3T3 cells were co-transfected with the Fshb mRNA 3′ UTR controlling Firefly luciferase reporter translation and either eGFP alone (peGFPN1), eGFP-Musashi1 (peGFP Msi1), or eGFP linked to an RNA binding mutant form of Musashi1 (peGFP Msi1-bm). Firefly luciferase enzyme activity values were normalized to control Renilla luciferase enzyme activity values (expressed from the same plasmid, FF/Renilla). Five separate experiments were performed with each condition measured in quadruplicate. Values that differ significantly after one-way ANOVA are indicated, ** (P value < 0.01), *** (P value < 0.001). A representative experiment is shown.