Table 1.
qPCR primers
| Gene name | IDT Assay ID | Ref Seq # | Forward sequence (5′ to 3′) | Reverse sequence (5′ to 3′) |
|---|---|---|---|---|
| Atf4 | Mm.PT.58.32663810.g | NM_009716 | GGCTATGGATGATGGCTTGG | ACAGAGCATCGAAGTCAAACTC |
| Avpr1b | Mm.PT.58.12605492 | NM_011924 | ACGAGAATGCCCCTAATGAAG | TGTTGAAGCCCATATAGATCCAG |
| Cdh1 | Mm.PT.58.43112087 | NM_009864 | ACAGCACATATGTAGCTCTCATC | CGTTGTCATTGACGTCTAACAG |
| Cldn4 | Mm.PT.58.11569298.g | NM_009903 | CACTCAGCCTACACGTTACTC | CACTCAGCACACCATGACTT |
| Creb3l2 | Mm.PT.58.42926069 | NM_178661 | AGTACTGTTGGAGCTTCAGC | AAAGGACGATCAGATGGAGTTAG |
| Cx3cl1 | Mm.PT.58.8767901 | NM_009142 | ATCCGCTATCAGCTAAACCAG | TGATCCAGATGCTTCATGGC |
| Cxcl2 | Mm.PT.58.8366748 | NM_001012477 | GCGCTCTGCATCAGTGA | ACAGTTTGGAGTGTTGAGGAT |
| Cxcl14 | Mm.PT.58.21980826 | NM_019568 | CAAGTGGTACAATGCCTGGA | CTGCTGAAGTCTCTCAACTGG |
| Ppia | Custom oligo (58) | NM_008907 | TGGTCTTTGGGAAGGTGAAAG | TGTCCACAGTCGGAAATGGT |
| Ddx6 | Mm.PT.58.730224 | NM_001110826 | CAATCTTGTTTGCACTGATCTGT | CAAGATGACCAAAGCGACCT |
| Foxj1 | Mm.PT.58.7661191 | NM_008240 | ATCACGGACAACTTCTGCTAC | GCACTTTGATGAAGCACTTGT |
| Foxo3 | Mm.PT.58.7873180 | NM_019740 | CTGAAGGATCACTGAGGAAAGG | GATCACTGTCCACTTGCTGAG |
| Foxp2 | Mm.PT.58.29755951 | NM_053242 | CGAACGTCTTCAAGCGATGA | CGACATGGTGACACTAGACAC |
| Fshb | Custom oligo (59) | GTGCGGGCTACTGCTACACT | CAGGCAATCTTACGGTCTCG | |
| Gata2 | Mm.PT.58.13913016.g | NM_008090 | CCTCTACTACAAGCTGCACAA | TCTTGGATTTGCTGGACATCT |
| Ghsr | Mm.PT.58.9588879 | NM_177330 | ACCAGAACCACAAACAGACAG | CAGGCTCGAAAGACTTGGAAA |
| Gjd2 | Mm.PT.58.6160898 | NM_010290 | AGCAGCACTCCACTATGATTG | GTTGCACACAAACATGGTCTG |
| Gnrhr | Custom Oligo (60) | NM_001003685.1 | GGCTGCCTCTTCATCCCCCT | CGTTCTCAGCCGAGCTCTTGGG |
| Insig1 | Mm.PT.58.9805181 | NM_153526 | CCCTGATTTCCTCTATATCCGTTC | ACTCTGAACCATGTGCTGAAG |
| Lhb | Custom Oligo (61) | TGTCCTAGCATGGTCCGAGT | AGGAAAGGAGACTATGGGGTCTA | |
| Msi1 | Mm.PT.58.31248964 | NM_008629(1) | CCTGCAAGATGTTCATCGGA | TGAAAGTGACGAAGCCGAAA |
| Msi2 | Custom Oligo (62) | GCGATGCTGATGTTCGACAA | TCTCCACAACGTCTTCATTCTCA | |
| Norad | Mm.PT.58.43986704.g | NR_024329 | CCTCTTGAACATGGAACTGCTA | AGACCTCATCCTGTGAACCT |
| Nr5a1 | Mm.PT.58.11115555 | NM_139051 | GAGTTAGTGCTCCAGTTGCAT | CTTTACGAGGCTGTGGTTGT |
| Ormdl1 | Mm.PT.58.10595788 | NM_145517 | GGACTACGGAGTACAGTTTACATC | TGGGAATAAGTACACTCAGCAG |
| Ormdl3 | Mm.PT.56a.42805026 | NM_025661 | CTGTCGTCTGGACCCTCA | TGAACTGGACCCCGTAGT |
| Pitx1 | Mm.PT.58.9006855 | NM_011097 | TGAGCATGAGAGAGGAGATCG | CCTTGCACAGGTCCAACT |
| Prop1 | Mm.PT.58.8919461 | NM_008936 | CTCAGTGAAGCCAGAATCCAG | GCAAGAAGCCAGAGAAGGT |
| Rab21 | Mm.PT.58.45878817 | NM_024454 | GCAGAATCGTATGCAGAGTCT | TTTTGCTCTCTCATCCACCTG |
| Rab27a | Mm.PT.58.7083493 | NM_023635 | GTGCTGTGTGGAAATAAGAGTG | GCTTATGTTTGTCCCGTTGG |
| Rab2a | Mm.PT.58.11823848 | NM_021518 | CGAGAGCATGGACTTATCTTCA | ATTTTAATGCCGTTTGCCTCA |
| Rab3d | Mm.PT.58.42963574 | NM_031874 | GACAATGCCCAGGTAATCCT | CACCTGCTTCACATTGATATTCTC |
| Rxra | Mm.PT.58.5331319 | NM_011305 | GAAGCGTACTGCAAACACAAG | CCCGATGAGCTTGAAGAAGA |
| Sox9 | Mm.PT.58.41323084 | NM_011448 | ACTCCCCACATTCCTCCT | CCCCTCTCGCTTCAGATCA |
| Sprtn | Mm.PT.56a.29073822 | NM_001111141 | GAAGACAAAAGCAGACAAGCAG | CGTCTCCAAGAACATAGCCTT |
| Tbx19 | Mm.PT.58.23718192 | NM_032005 | ATCCTGTCTGTACCCCACA | AGAATTGTTCCTGAGGTGCTG |
| Yap1 | Mm.PT.58.10478408 | NM_001171147 | TGACAACCAATAGTTCCGATCC | CCTGTATCCATTTCATCCACACT |
Abbreviation: IDT, Integrated DNA Technologies, Inc.