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Antibody variants of the anti-Fn14-antibody 18D1. (A) Scheme of the domain architecture of the various 18D1 variants used in this study. (B, C) Western blot analysis of 10 µl supernatant of HEK293 cells transiently expressing the proteins shown in (A). Samples in (C) were dissolved in Laemmli buffer without β-mercaptoethanol, thus without reduction.
