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. Author manuscript; available in PMC: 2023 Aug 4.
Published in final edited form as: Cell Rep. 2023 Apr 26;42(5):112436. doi: 10.1016/j.celrep.2023.112436

Figure 1. PSGL-1 restrains TCR signaling.

Figure 1.

(A) Frequencies of OT-I WT and PSGL-1−/− CD8+ T cells expressing activation markers at 18 h stimulation with anti-CD3ε antibody. Each dot represents an individual mouse. Experiments were performed 2×.

(B) Western blot of phosphorylated and total levels of Zap70, Erk1/2, AKT, and GAPDH in WT and PSGL-1−/− OT-I cells stimulated for the indicated time with anti-CD3ε antibody.

(C) Relative levels of phosphorylated Zap70, Erk1/2, and AKT, normalized to β-actin and relative to the specific protein of interest value in WT samples at 0 min for each blot.

Experiments were performed 3×, 3 mice pooled/genotype, per experiment. (A–C) All data are parametric data except pAkt at 5 min. For parametric data, unpaired t tests were performed; Mann-Whitney test used for non-parametric data. Error bars are SEM. *p < 0.05, **p < 0.01, ***p < 0.005.

See also Figure S1.