| Antibodies |
|
| BUV395 Mouse Anti-Human CD4 |
BD Biosciences |
Cat# 563550; RRID: AB_2738273 |
| BUV395 Rat Anti-Mouse CD8a |
BD Biosciences |
Cat# 563786; RRID: AB_2732919 |
| BUV496 Mouse Anti-Human CD8 |
BD Biosciences |
Cat# 612942; RRID: AB_2870223 |
| BUV737 Mouse Anti-Human CD45RA |
BD Biosciences |
Cat# 612846; RRID: AB_2870168 |
| BUV737 Mouse Anti-Mouse CD45.1 |
BD Biosciences |
Cat# 612811; RRID: AB_2870136 |
| BUV737 Rat Anti-Mouse CD11a |
BD Biosciences |
Cat# 741727; RRID: AB_2871097 |
| BUV737 Rat Anti-Mouse CD223 |
BD Biosciences |
Cat# 741820; RRID: AB_2871155 |
| BV421 Mouse Anti-Mouse CD244.2 |
BD Biosciences |
Cat# 740015; RRID: AB_2739787 |
| BV421 Rat Anti-Mouse CD244.1 |
BD Biosciences |
Cat# 744284; RRID: AB_2871568 |
| BV480 Mouse Anti-Human CD44 |
BD Biosciences |
Cat# 746479; RRID: AB_2743781 |
| BV605 Mouse Anti-Mouse Ly-108 |
BD Biosciences |
Cat# 745250; RRID: AB_2742834 |
| R718 Rat Anti-Human CCR7 (CD197) |
BD Biosciences |
Cat# 751861 |
| R718 Rat Anti-Mouse CD4 |
BD Biosciences |
Cat# 566939; RRID: AB_2869957 |
| Alexa Fluor® 647 anti-human CD101 (BB27) |
BioLegend |
Cat# 331010; RRID: 2562145 |
| APC/Fire™ 750 anti-human CD45RO |
BioLegend |
Cat# 304250; RRID: AB_2616717 |
| APC/Fire™ 750 anti-mouse CD38 |
BioLegend |
Cat# 102738; RRID: AB_2876402 |
| Apotracker™ Green |
BioLegend |
Cat# 427403 |
| Brilliant Violet 421™ anti-human CD38 |
BioLegend |
Cat# 303526; RRID: AB_10900230 |
| Brilliant Violet 605™ anti-human CD25 |
BioLegend |
Cat# 302632; RRID: AB_11218989 |
| Brilliant Violet 605™ anti-rat CD90/mouse CD90.1 (Thy-1.1) |
BioLegend |
Cat# 202537; RRID: AB_2562644 |
| Brilliant Violet 711™ anti-human CD366 (Tim-3) |
BioLegend |
Cat# 345024; RRID: AB_2564046 |
| Brilliant Violet 711™ anti-mouse CD366 (Tim-3) |
BioLegend |
Cat# 119727; RRID: AB_2716208 |
| Brilliant Violet 785™ anti-human CD223 (LAG-3) |
BioLegend |
Cat# 369322; RRID: AB_2716127 |
| Brilliant Violet 785™ anti-mouse CD279 (PD-1) |
BioLegend |
Cat# 135225; RRID: AB_2563680 |
| PE/Cyanine7 anti-mouse CD25 |
BioLegend |
Cat# 101916; RRID: AB_2616762 |
| APC anti-mouse CD69 |
BioLegend |
Cat# 104514; RRID: AB_492843 |
| BUV496 Rat Anti-Mouse CD44 |
BD Bioscience |
Cat# 741057; RRID: AB_2870671 |
| Brilliant Violet 421™ anti-mouse CX3CR1 |
BioLegend |
Cat# 149023; RRID: AB_2565706 |
| FITC anti-mouse CD45.1 |
BioLegend |
Cat# 110706; RRID: AB_313495 |
| FITC anti-mouse CD49d |
BioLegend |
Cat# 103606; RRID: AB_313037 |
| Human TruStain FcX™
|
BioLegend |
Cat# 422302; RRID: AB_2818986 |
| PE anti-human CD39 |
BioLegend |
Cat# 328208; RRID: AB_940429 |
| PE anti-mouse CD160 |
BioLegend |
Cat# 143004; RRID: AB_10960743 |
| PE/Cyanine7 anti-human CD160 |
BioLegend |
Cat# 341212; RRID: AB_2562876 |
| PE/Dazzle™ 594 anti-human CD279 (PD-1) |
BioLegend |
Cat# 367434; RRID: AB_2783284 |
| PE/Dazzle™ 594 anti-mouse CD62L |
BioLegend |
Cat# 104448; RRID: AB_2566163 |
| PE/Dazzle™ 594 anti-mouse CD90.2 (Thy-1.2) |
BioLegend |
Cat# 140329; RRID: AB_2650965 |
| PerCP/Cyanine5.5 anti-human CD69 |
BioLegend |
Cat# 310926; RRID: AB_2074956 |
| PerCP/Cyanine5.5 anti-mouse CD107a (LAMP-1) |
BioLegend |
Cat# 121626; RRID: AB_2572055 |
| PerCP/Cyanine5.5 anti-mouse CD223 (LAG-3) |
BioLegend |
Cat# 125212; RRID: AB_2561517 |
| Purified anti-mouse CD16/32 |
BioLegend |
Cat# 101302; AB_312801 |
| CD101 PECy7 |
eBioscience/ThermoFisher |
Cat# 25–1011-82; RRID: AB_2573378 |
| H2-D(b) GP(33–41) APC or BV421 Tetramer |
NIH Tetramer Core |
N/A |
| H2-D(b) NP(396–401) BV421 Tetramer |
NIH Tetramer Core |
N/A |
| PE Mouse Anti-TCF-7/TCF-1 |
BD Biosciences |
Cat# 564217; RRID: AB_2687845 |
| PE Mouse Anti-TCF-7/TCF-1 |
BD Biosciences |
564217 |
| APC anti-human TNF-α |
BioLegend |
Cat# 502912; RRID: AB_315264 |
| APC anti-mouse IFN-γ |
BioLegend |
Cat# 505810; RRID: AB_315404 |
| APC anti-mouse Perforin |
BioLegend |
Cat# 154304; RRID: AB_2721463 |
| Brilliant Violet 421™ anti-human IFN-γ |
BioLegend |
Cat# 502532; RRID: AB_2561398 |
| Brilliant Violet 421™ anti-human/mouse Granzyme B |
BioLegend |
Cat# 396414; RRID: AB_2810603 |
| Brilliant Violet 421™ anti-T-bet |
BioLegend |
Cat# 644816; RRID: AB_10959653 |
| PE anti-human IL-2 |
BioLegend |
Cat# 500307; RRID: AB_315094 |
| PE anti-mouse IL-2 |
BioLegend |
Cat# 503808; RRID: AB_315302 |
| PE/Cyanine7 anti-mouse TNF-α |
BioLegend |
Cat# 506324; RRID: AB_2256076 |
| Alexa Fluor 647 Mouse Anti-ZAP70 |
BioLegend |
Cat# 691205; RRID: AB_2721438 |
| Brilliant Violet 421™ anti-Mouse CD3 |
BioLegend |
Cat# 100228; RRID: AB_2562553 |
| CD162 (PSGL-1) PE |
eBioscience/ThermoFisher |
Cat# 12–1621-82; RRID: AB_2848256 |
| EOMES PECy7 |
eBioscience/ThermoFisher |
Cat# 25–4875-82; AB_2573454 |
| EOMES PerCP-eFluor710 |
eBioscience/ThermoFisher |
Cat# 46–4877-42; RRID: AB_2573759 |
| Ki67 PerCP-eFluor710 |
eBioscience/ThermoFisher |
Cat# 46–5698-82; RRID: AB_11040981 |
| T-bet eFluor 450 |
eBioscience/ThermoFisher |
Cat# 48–5825-82; RRID: AB_2784727 |
| TOX eFluor 660 |
eBioscience/ThermoFisher |
Cat# 50–6502-82; RRID: AB_2574265 |
| UBASH3B/STS 1 polyclonal Rabbit IgG antibody |
Proteintech |
Cat# 19563–1-AP; RRID: AB_10643379 |
| B-actin Mouse mAb |
Cell Signaling |
Cat# 3700; RRID: AB_2242334 |
| IRDye800CW Goat anti-Rabbit IgG Secondary Antibody |
Licor |
Cat# 926–32211; RRID: AB_621843 |
| IRDye680RD Goat anti-Mouse IgG Secondary Antibody |
Licor |
Cat# 926–68070; RRID: AB_10956588 |
| Zap70 Rabbit mAb |
Cell Signaling |
Cat# 2705; RRID: AB_2273231 |
| Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Rabbit mAb |
Cell Signaling |
Cat# 2717; RRID: AB_2218658 |
| Akt (pan) Mouse mAb |
Cell Signaling |
Cat# 2920; RRID: AB_1147620 |
| Phospho-Akt (Ser473) Rabbit mAb |
Cell Signaling |
Cat# 9271; RRID: AB_329825 |
| p44/42 MAPK (Erk1/2) Mouse mAb |
Cell Signaling |
Cat# 4696; RRID: AB_390780 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Thyr204) XP Rabbit mAb |
Cell Signaling |
Cat# 4370; RRID: AB_2315112 |
| Fab anti-mouse CD162 (PSGL-1) |
mAb from BioXcell, Fab fragments custom made by Ab Lab (University of Vancourer, BC) |
mAb: Cat# BE0186; RRID: AB_10950305 |
| Anti-mouse CD3e |
BioXCell |
Cat# BE0001–1; RRID: AB_1107634 |
| Anti-mouse CD28 |
BioXCell |
Cat# BE00015–1 |
| ImmunoCult Human CD3/CD28 T Cell Activator |
StemCell |
Cat# 10971 |
|
| Bacterial and virus strains |
|
| LCMV Clone 13 |
This lab |
N/A |
|
| Biological samples |
|
| Human PBMCs, melanoma patients |
University of California San Diego, Moore's Cancer Center |
N/A |
| Human PBMCs, healthy controls |
iXCells |
10HU-003-CR50M |
|
| Chemicals, peptides, and recombinant proteins |
|
| SIINFEKL (OVA, N4) |
GenScript, Custom Order |
N/A |
| SIIQFEKL (OVA, Q4) |
GenScript, Custom Order |
N/A |
| SIITFEKL (OVA, T4) |
GenScript, Custom Order |
N/A |
| SIIVFELK (OVA, V4) |
GenScript, Custom Order |
N/A |
| REAGENT or RESOURCE |
SOURCE |
IDENTIFIER |
| KAVYNFATM (LCMV GP33–41) |
GenScript, Custom Order |
N/A |
| FQPQNGQFI (LCMV NP396–404) |
GenScript, Custom Order |
N/A |
| Recombinant PSGL-1 |
This lab, Aragen Life Sciences |
N/A |
|
| Critical commercial assays |
|
| eBioscience Foxp3/Transcription Factor Staining Buffer Set |
ThermoFisher |
00–5523-00 |
| 2-NBDG |
Cayman Chemical |
11046 |
| Miltenyi Mouse Tumor Dissociation Kit |
Miltenyi Biotec |
130–096-730 |
| APEX AF488 Microscale Protein Labeling Kit |
ThermoFisher |
A30006 |
| Seahorse Glycolytic Rate Assays |
Agilent Technologies |
103346–100 |
| 10X Single Cell 3' Reagent Kits v2 |
10X Genomics |
Multiple |
|
| Deposited data |
|
| WT vs PSGL-1-/- CD8+ T cells, LCMV Cl13 |
Reference #6 (Tinoco) |
GEO: GSE80113
|
| Epigenetics of CD8+ T cells during differentiation |
Reference #59 (Yu) |
GEO: GSE89036
|
| Raw and analyzed scRNA-seq data |
This paper |
GEO: GSE226523
|
| Raw and analyzed ATAC-seq data |
This paper |
GEO: GSE226521
|
|
| Experimental models: Cell lines |
|
| YUMM1.5 melanoma |
Marcus Bosenburg |
N/A |
| B16-OVA melanoma |
Ananda Goldrath |
N/A |
| TK-1 T cell lymphoblast |
ATCC |
CRL-2396 |
|
| Experimental models: Organisms/strains |
|
| C57BL/6J |
The Jackson Laboratory |
000664 |
| B6.Cg-Selplgtm1Fur/J (PSGL-1-/-) |
The Jackson Laboratory |
004201 |
| C57BL/6 Tg(TcraTcrb)1100Mjb/J (OT-I) |
The Jackson Laboratory |
003831 |
| B6.Cg-Tcratm1MomTg(TcrLCMV)327Sdz/TacMmjax (P14) |
The Jackson Laboratory |
025408 |
| B6.SJL-PtprcaPepcb/BoyJ (CD45.1) |
The Jackson Laboratory |
000686 |
| B6.PL-Thy1a/CyJ (CD90.1/Thy1.1) |
The Jackson Laboratory |
000406 |
|
| Software and algorithms |
|
| FlowJo v10 |
FlowJo, BD |
N/A |
| INSPIRE V200.1.388.0 |
Amnis |
N/A |
| IDEAS V6.2.183.0 |
Amnis |
N/A |
| SeqGeq |
FlowJo, BD |
N/A |
| WAVE |
Agilent Technologies |
N/A |
| GraphPad Prism 9 |
GraphPad |
N/A |
| MSstats TMT Bioconductor |
Bioconductor |
N/A |
| IntegrateData |
Seurat V4.1.1. |
N/A |
| RunUMAP |
Seurat V4.1.1. |
N/A |
| FindClusters |
Seurat V4.1.1. |
N/A |
| FindAllMarkers |
Seurat V4.1.1. |
N/A |
| DoHeatmap |
Seurat V4.1.1. |
N/A |
| VlnPlot |
Seurat V4.1.1. |
N/A |
| Stat_compare_means |
ggpubr V0.4.0. |
N/A |
| IGV Genome Browser V2.9.2. |
Broad Institute |
N/A |
| IPA Pathway Analysis |
Qiagen |
N/A |
| 10X Genomics Cell Ranger V2.2.0. |
10X Genomics |
N/A |
| MaxQuant Software 1.6.11.0 |
MaxQuant |
N/A |
