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. 2023 Jul 25;17(7):e0011520. doi: 10.1371/journal.pntd.0011520

Fig 5. Inhibition Zbtb7a by mmu-miR-342-3p via directly binding to its 3′UTR.

Fig 5

(A) Schematic representation of a putative binding site of mmu-miR-342-3p in the 3’UTR of Zbtb7a (up) and the dual-Glo Luciferase assay for the interaction between mmu-miR-342-3p-Mimics and Zbtb7a (down). The miRNA ‘seed’ sequences are indicated in bold in black. WT: WT- Zbtb7a or Mut- Zbtb7a construct was co-transfected into 293T cells with either mimic negative control (mimic NC) or mmu-miR-342-3p mimic (miR-342-3p mimic) and luciferase activities were measured after 24 h transfection. (B) The expression of mmu-miR-342-3p in the HSCs from E. multilocularis-infected mice transfected with inhibitor negative control (NC) or mmu-miR-342-3p inhibitor (Inhibitor) by RT-qPCR. (C) The expression of Zbtb7a at mRNA and protein levels in the HSCs from E. multilocularis-infected mice transfected with mmu-miR-342-3p inhibitor (Inhibitor). (D) The expression of zbtb7a in primary HSCs from E. multilocularis-infected mice at 30-, 60-, and 90-day post-infection (dpi). (E) Correlation between the expression of Zbtb7a and mmu-miR-342-3p. (n = 9, Spearman’s correlation analysis; r, correlation coefficient) Data for final statistical analysis were taken from 3 independent experiments. **p < 0.01; ***p < 0.001.