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. 2023 Jul 20;19(7):e1011517. doi: 10.1371/journal.ppat.1011517

Fig 6. Most of the candidate ETC inhibitors target the ETC upstream of cytochrome c in P. falciparum parasites.

Fig 6

(A) Schematic of the assay measuring the O2 consumption rate (OCR) of permeabilized P. falciparum parasites supplied malate (Mal) as a substrate. The following addition of substrates and inhibitors were performed: Port A, the test compound; Port B, TMPD; Port C, sodium azide (NaN3). CoQ, coenzyme Q; III, Complex III; CytC, cytochrome c; IV, Complex IV; e-, electrons. (B-G) Traces depicting parasite OCR over time when supplying Mal as a substrate. The candidate ETC inhibitors tested (all at 10 μM) were (B) atovaquone, (C) auranofin, (D) trifloxystrobin, (E) azoxystrobin, (F) MMV024397, or (G) MMV688853. Values represent the mean ± SD of three technical replicates and are representative of three independent experiments; error bars that are not visible are smaller than the symbol. Dotted lines represent the time points of each injection, and data points for each condition have been connected by lines to aid interpretation.