Table 4. O2 consumption rate inhibitory activity of MMV688853 against drug-resistant T. gondii strains.
Determination of the O2 consumption rate (OCR) inhibitory properties of MMV688853 against T. gondii parasite strains resistant to atovaquone (ATVR ME49) or ELQ-300 (ELQR RH) and the corresponding ATV-sensitive (WT ME49) and ELQ-300-sensitive (ELQS) parental strains. Data are reported as average EC50OCR (μM) ± SEM from three independent experiments. EC50OCR values against atovaquone and ELQ-300 were determined as controls in each strain. FC, fold-change in EC50OCR values between the atovaquone-resistant strain and corresponding atovaquone-sensitive parental strain. Paired t-tests were performed to compare the EC50OCR of the compounds in WT vs ATVR parasites, and p-values are depicted as ns = not significant (p > 0.05), * p < 0.05.
| WT ME49 | ATVR ME49 | ELQS RH | ELQR RH | ||
|---|---|---|---|---|---|
| Compound | EC50OCR (μM) | EC50OCR (μM) | FC | EC50OCR (μM) | EC50OCR (μM) |
| MMV688853 | 1.9 ± 0.1 | 1.5 ± 0.1 | 0.8 * | 4.0 ± 0.7 | > 40 |
| Atovaquone | 0.027 ± 0.006 | 1.4 ± 0.3 | 52 * | 0.15 ± 0.02 | 0.09 ± 0.02 |
| ELQ-300 | 0.60 ± 0.1 | 0.52 ± 0.1 | 0.9 ns | 2.8 ± 0.7 | > 4 |