Fig. 1. An integrative strategy for identifying functional lncRNAs in GBM with clinically relevant expression.

(A) Workflow diagram depicting the integrative strategy for identifying GBM-associated lncRNAs and designing CRISPRi sgRNA library. (B) Schema of the workflow for construction of lentiviral vectors encoding sgRNA library and experimental design of CRISPRi screens. The scatterplot showing the statistical significance −log₁₀ (P value) and log₂ (fold change) [log₂(FC)] between D21 and D0, for the representative negatively selected sgRNA of the corresponding lncRNA genes in (C) U87 and (D) U251 cells. The lncRNA genes with zero, one, and at least two significantly depleted sgRNAs are colored in gray, blue, and yellow in the indicated cell lines, respectively. The lncRNA genes with at least two significantly depleted sgRNAs in both cell lines are shown in red. (E) Venn diagram showing the overlap of the lncRNA genes with at least two significantly negatively selected targeting sgRNAs in U251 and U87.