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. 2023 Aug 4;9(31):eadf3984. doi: 10.1126/sciadv.adf3984

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Fig. 7. — The representative images of nuclear γ-H2AX foci detected by the immunofluorescence staining with anti–γ-H2AX (green) in the DAPI-stained nuclei (blue) from (A) U251 cells transfected with the negative control siRNA (si-NC) or the DARS1-AS1/YBX1–targeting siRNAs or from (B) GSC11 cells transduced with the negative control shRNA (sh-NC) or the DARS1-AS1/YBX1–targeting shRNAs. Scale bars, 24 μm. The percentages of γ-H2AX foci–positive cells (>5 foci in the nucleus) were shown as bar plots for the indicated conditions. The effect of siRNA-mediated knockdown of (C) DARS1-AS1 or (D) YBX1 compared with si-NC on the HR repair efficiency was assessed in U251 cells using a DR-GFP assay (Materials and Methods). Data in (A) to (D) are shown as mean ± SD (n = 3). **P < 0.01 by one-way ANOVA with Dunnett’s multiple comparison test.