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. 2023 Aug 4;9(31):eadf3984. doi: 10.1126/sciadv.adf3984

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Fig. 8. — RT-qPCR analysis of the RNA level of the known regulators for HR-mediated DSB repair FOXM1, RAD51, and BRCA1 in (A) U251 and (B) GSC11 cells transfected/transduced with the negative control si-NC/sh-NC or individual siRNAs/shRNAs targeting DARS1-AS1. The protein level of FOXM1, RAD51, and BRCA1 as well as the γ-H2AX level was determined by Western blotting in (C) U251 and (D) GSC11 cells with/without RNAi-mediated DARS1-AS1 depletion. RT-qPCR analysis of the RNA level of HR-mediated DSB repair–related genes FOXM1, RAD51, and BRCA1 in (E) U251 and (F) GSC11 cells transfected/transduced with the negative control si-NC/sh-NC or individual siRNAs/shRNAs targeting YBX1. The protein level of FOXM1, RAD51, and BRCA1 as well as the γ-H2AX level were determined by Western blotting in (G) U251 and (H) GSC11 cells with/without RNAi-mediated YBX1 depletion. (I) Representative pictures of clonogenic growth and (J) bar graph quantifying the colonies formed by U251 cells that were transduced with the sh-NC or shRNAs targeting DARS1-AS1. (K) Representative pictures of soft agar colony formation and (L) bar graph quantifying the colonies formed in soft agar by GSC11 cells that were transduced with the sh-NC or shRNAs targeting DARS1-AS1. Scale bar, 500 μm. Data in (A), (B), (E), (F), (J), and (L) are shown as mean ±SD (n = 3). **P < 0.01 by one-way ANOVA with Dunnett’s multiple comparison test.