Figure 2. Puerarin reduced oxidative stress and ferrotosis induced by I/R in rats. Different concentration of puerarin (50 and 100 mg/kg) was intraperitoneally injected before renal I/R model established, once a day for seven days, and the sham group was injected with the same amount of physiological saline. Then, the rats in I/R group was underwent ischemia for 45 minutes and reperfusion 12 weeks. (a) The measurement of SOD expression in each group; (b) the measurement of MDA expression in each group; (c) the measurement of GSH expression in each group; (d) the measurement of normalized Fe²⁺ level in each group (n = 8). All data were showed as mean ± standard error mean, and two-way analysis of variance was used. Each experiment was repeated at least three times independently.

SOD: superoxide dismutase; MDA: malondialdehyde; GSH: glutathione; I/R: ischemia and reperfusion; *P < 0.05 vs. sham group; #P < 0.05 vs. I/R group.