Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Spodoptera frugiperda) | Sf9 | Other | Cell culture facility at UC Berkeley | |
| Cell line (Homo sapiens) | HEK 293T cells | Other | Cell culture facility at UC Berkeley | |
| Cell line (Homo sapiens) | HEK 293T-YBX1 KO cells | Other | Obtained by CRISPR-Cas9 in Schekman Lab | |
| Cell line (Homo sapiens) | HEK 293T-YBAP1 KO | This study | Obtained by CRISPR-Cas9 in Schekman Lab | |
| Cell line (Homo sapiens) | HEK 293T-3xHA-EGFP-OMP25 | This study | Obtained by overexpression of pLJM1-3XHA-EGFP-OMP25 in Schekman lab | |
| Cell line (Homo sapiens) | U-2OS cells | Other | Cell culture facility at UC Berkeley | |
| Cell line (Homo sapiens) | U-2OS Parkin-GFP cells | This study | Obtained by overexpression of Parkin-GFP in Schekman lab | |
| Recombinant DNA reagent | pFastBac His6 MBP N10 TEV LIC cloning vector (4 C) | Addgene | RRID: Addgene_30116 | N/A |
| Recombinant DNA reagent | Tom22-mCherry (plasmid) | This study | Gift of Dr Li Yu lab | |
| Recombinant DNA reagent | His-MBP-YBX1 (plasmid) | This study | To express YBX1 in insect cells. Plasmid maintained in Schekman lab | |
| Recombinant DNA reagent | His-MBP-YBX1(1–51) (plasmid) | This study | To express YBX1(1–51) in insect cells. Plasmid maintained in Schekman lab | |
| Recombinant DNA reagent | His-MBP-YBX1(52–129) (plasmid) | This study | To express YBX1(52–129) in insect cells. Plasmid maintained in Schekman lab | |
| Recombinant DNA reagent | His-MBP-YBX1(130–324) (plasmid) | This study | To express YBX1(1–51) in insect cells. Plasmid maintained in Schekman lab | |
| Recombinant DNA reagent | His-MBP-YBX1(1–129) (plasmid) | This study | To express YBX1(1–129) in insect cells. Plasmid maintained in Schekman lab | |
| Recombinant DNA reagent | His-MBP-YBX1(F85A) (plasmid) | This study | To express YBX1(F85A) in insect cells. Plasmid maintained in Schekman lab | |
| Recombinant DNA reagent | His-MBP-YBAP1 (plasmid) | This study | To express YBAP1 in insect cells. Plasmid maintained in Schekman lab | |
| Recombinant DNA reagent | Mito-BFP | This study | Gift of Dr. Samantha Lewis lab | |
| Recombinant DNA reagent | mCherry-Rab5(Q79L) (plasmid) | Addgene | RRID: Addgene_35138 | |
| Recombinant DNA reagent | pLJM1-3XHA-EGFP-OMP25 | This study | To express 3xHA-EGFP-OMP25 in HEK293T cells. Plasmid maintained in Schekman lab | |
| Antibody | Anti-YBX1 (Rabbit polyclonal) |
Abcam | RRID: AB_1950384 | WB 1:1000 |
| Antibody | Anti-YBAP1 (Mouse monoclonal) |
Santa Cruz | RRID: AB_10611471 | WB 1:1000 |
| Antibody | Anti-YBAP1(Rabbit polyclonal) | Thermo Fisher Scientific | RRID: AB_2638956 | WB 1:1000 |
| Antibody | Anti-Tim23 (Mouse monoclonal) | BD Biosciences | RRID: AB_398754 | WB 1:1000 |
| Antibody | Anti-Tom20 (Mouse monoclonal) | Abcam | RRID: AB_945896 | WB 1:1000 |
| Antibody | Anti-Calnexin (Rabbit polyclonal) | Abcam | RRID: AB_2069006 | WB 1:2000 |
| Antibody | Anti-HA (Rabbit monoclonal) | Cell Signaling | RRID: AB_1549585 | WB 1:1000 |
| Antibody | Anti-COX IV (Rabbit Monoclonal) | Cell signaling | RRID: AB_2085424 | WB 1:1000 |
| Antibody | Anti-Citrate Synthase (Rabbit monoclonal) | Cell signaling | RRID: AB_2665545 | WB 1:1000 |
| Antibody | Anti-Rab5 (Rabbit monoclonal) | Cell signaling | RRID: AB_2300649 | WB 1:1000 |
| Antibody | Anti-LAMP1 (Rabbit monoclonal) | Cell signaling | RRID: AB_2687579 | WB 1:1000 |
| Antibody | Anti-GRP78 (Rabbit polyclonal) | Abcam | RRID: AB_2119834 | WB 1:3000 |
| Antibody | Anti-GADPH (Rabbit monoclonal) | Cell signaling | RRID: AB_561053 | WB 1:5000 |
| Antibody | Anti-alpha Tubulin (Mouse monoclonal) | Abcam | RRID: AB_2241126 | WB 1:5000 |
| Antibody | Anti-beta Actin (Mouse monoclonal) | Abcam | RRID: AB_449644 | WB 1:5000 |
| Software, algorithm | Image Studio Lite | LICOR | https://www.licor.com/bio/image-studio-lite/ | |
| Software, algorithm | FIJI | NIH | RRID: SCR_002285 | https://fiji.sc/ |
| Software, algorithm | Prism 9 | GraphPad | RRID: SCR_002798 | https://www.graphpad.com |