Immunoblot of Env proteins expressed by EIAV-infected EK cells and retroviral vector-transduced EK cells reacted with MAb 30/8.12 (A) and MAb EIA6A1 (B). (A) Lysates from normal (lane 1), EIAV-infected (lane 2), vLXSN-transduced (lane 3), vLGSN-transduced (lane 4), and vLGP90SN-transduced (lane 5) EK cells. (B) Lysates from normal (lane 1), EIAV-infected (lane 2), recombinant vaccinia virus (VGag/Pr)-infected (lane 3), vLXSN-transduced (lane 4), vLGSN-transduced (lane 5), and vLGP90SN-transduced (lane 6) EK cells. Sizes are indicated in kilodaltons.