Figure S3.

cVA on a freely moving male conveys positional information that can be used for lateralized behavior, related to Figure 3

(A) Relative locations of stimulus male upon turn start by receiver fly, rotated to receiver coordination system. Related to Figure 3A. Left: females, n = 32 pairs per condition, with the right non-olfactory arista cut (control, the left in each pair) or right antenna cut (ablation, the right in each pair). Right: males, 24 pairs per condition (each pair consisted of two manipulated males; overall, 48 manipulated males per group). Middle: detection range. Outer circle is 5 mm between the receiver fly antennae and the stimulus male abdomen. Inner circle is 2 mm between centroids of both flies; this area is excluded.

(B) Summed ORN GCaMP6f responses in the right and left DA1 glomerulus and the male’s distance inverted over time, rows are individual flies, 5-min recordings. The third fly from the top was shown in Figure 3C. Gaps in the traces correspond to periods when the male’s abdomen was facing toward the camera and away from the female, and therefore the male’s wings were in between the female’s antennae and the male’s abdomen.

(C) Same as (B) for DA1 lPN responses.

(D) Velocity traces along three axes (forward, lateral, rotation). Timing corresponds to the lateral displacement traces shown in Figure 3F, single trials following right (blue) and left (red) male presentations. Top row: forward velocity, middle row: lateral velocity, bottom row: rotational velocity. Three conditions shown from left to right in this order: a male fly presented with ambient light on, a dummy fly presented in the dark, a male fly presented in the dark.