Figure S6.

Third-order neurons extract distinct features of a male from cVA, related to Figure 6

(A) cVA PNs target a large and diverse array of third-order neurons. Downstream cell types of DA1 lPN (top) and DA1 lvPN (bottom) in the hemibrain, ordered by the absolute number of inputs (shown for every cell type) from the respective PN type. See STAR Methods for inclusion criteria. Number of cell types per class shown in the bottom right table. Colors represent broad cell-type classes. Magenta, multimodal local neuron (LN); purple, olfactory output neuron (ON); green, multimodal ON; turquoise, DA1-selective ON; orange, olfactory LN; yellow, multimodal descending neuron (DN). ACh, acetylcholine; GABA, γ-aminobutyric acid; Glu, glutamate. Scale bars, 40 μm. See also Table S4.

(B) Confocal image of AV2a2 in a female brain, reporter expression driven by 53A03-GAL4, (used in calcium imaging experiments), maximum projection. Scale bars, 20 μm.

(C) Confocal image of AV2a2 in a female and a male brain, reporter expression driven by AV2a2 SS, (used in behavior experiments), maximum projection. Scale bars, 20 μm.

(D) EM morphology of AV2a2, AD1g1, LPLC2, and LC4 neurons in the hemibrain dataset. Their schematic connectivity is shown in Figure 6K. Scale bars, 20 μm.

(E) GCaMP6f responses in females to a male stimulus (solid line), and in females to a virgin female stimulus (dotted line) in AV2a2 axons. Responses to a male are also shown in Figure 6G. n = 6, 6 trials, gray area is SEM.