REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
mouse anti-nc82 antibody	DSHB	RRID: AB_2314866
chicken anti-GFP antibody	Abcam	RRID: ab13970
mouse anti-ChAT4B antibody	DSHB	RRID: AB_528122
rabbit anti-GABA antibody	Sigma	RRID: A2052
goat Alexa-568 anti-mouse	Invitrogen	RRID: A21144
goat Alexa-488 anti-chicken	Invitrogen	RRID: A11038
goat Alexa-647 anti-mouse	Invitrogen	RRID: A21240
goat Alexa-568 anti-rabbit	Invitrogen	RRID: A11036
Experimental models: Organisms/strains
Canton-S strain	Jefferis lab, originated in Martin Heisenberg lab	CSMH
lPN-SS: w[1118]; GMR54A11-AD; BJD_115F09	gift from Dr Yoshinori Aso	SS01189
lvPN-SS: w[1118]; GMR38D01-AD; GMR59G08-DBD	gift from Dr Mike Dolan	LH467
yw, UAS-mCD8-GFP, UAS-mCD8-GFP	MRC Laboratory of Molecular Biology	N/A
w[1118];20XUAS-opGCaMP6s su(Hw)attP5;	gift from Yonil Jung,Barrett Pfeiffer, and David Anderson	N/A
w[1118];20XUAS-opGCaMP6f su(Hw)attP5;	gift from Yonil Jung,Barrett Pfeiffer, and David Anderson	N/A
w, Or67d-GAL4;;	BDSC	RRID: BDSC_9998
lPN GAL4 w[1118];; GMR24A10-GAL4	BDSC	RRID: BDSC_49059
il3LN6 GAL4 w[1118];; VT046100-GAL4	BDSC	RRID: BDSC_75076
W; UAS-Ort;	Liu and Wilson33	N/A
VT033066-LexA	Cachero et al.72	N/A
w[1118];; GMR53A03-GAL4	BDSC	RRID: BDSC_38858 (currently not available)
pC1-SS2: w[1118]; VT002064-p65ADZp in attP2, dsx-DBD	Wang et al.34	Janelia FlyLight SS59911
w[1118], 13XLexAop-IVS-jGCaMP7f su(Hw)attP8;;	BDSC	RRID: BDSC_80910
w;; dsx-LexA	Deutsch et al.73	N/A
w[1118]; 20XUAS-CsChrimson-mVenus;	BDSC	RRID: BDSC_55135
w[1118], UAS-Hid, UAS-Reaper;;	Wang et al.34	N/A
AV2a2-SS: w[1118]; GMR30A10-AD; GMR53A03-DBD	Dolan et al.35	LH907
aSP-g-SS1:w[1118];; GMR76G09-DBD, JK1029-AD	this study	N/A
w[1118]; P{y[+t7.7] w[+mC]=20XUAS-IVS-GCaMP6f}attP40	BDSC	RRID: BDSC_42747
yw, Or67d-QF;;	Riabinina and Potter74	N/A
;QUAS-Kir;	gift from Chris Potter	N/A
w[1118];; Ir94e-GAL41	Koh et al.45	N/A
w[1118];; Ir94e-GAL42	Sánchez-Alcañiz et al.46	N/A
w[1118]; PBac{y[+t7.7] w[+mC]=20XUAS-IVS-jGCaMP7f}VK00005	BDSC	RRID: BDSC_79031
w[1118]; GMR81A04-LexA;	BDSC	RRID: BDSC_54390
aSP-g-SS1: w[1118];;GMR76G09-DBD,JK1029	this study	N/A
aSP-g-SS1/aSP-g-SS2: w[1118];;GMR76G09-DBD,JK1029/GMR81A04-DBD,JK1029	this study	N/A
JK1029, empty-DBD: w[1118]; JK1029,GAL4-DBD.empty control (BPZpGDBD)	this study	N/A
w[1118];; fruFLP	Von Philipsborn et al.75	N/A
w[1118];;20XUAS> myrTopHat2 > GCaMP6f su(Hw)attP1	gift from Yonil Jung,Barrett Pfeiffer, and David Anderson	N/A
aSP-g-FLP1: w[1118];; 76G09-GAL4, fruFLP	this study	N/A
aSP-g-FLP2: w[1118];; 81A04-GAL4, fruFLP	this study	N/A
empty-GAL4 FLP: w[1118];; empty-GAL4 (pBDPGAL4Uw), fruFLP	this study	N/A
w;; UAS>mCherry>eGFP:Kir2.1	Watanabe et al.76	N/A
aSP-g LexA FLP: w[1118];; 81A04-LexA, fruFLP	this study	N/A
13xLexAop>dsFRT>CsChrimson:mVenus;;	Gift from Yoshinori Aso	N/A
w;; 20xUAS>dsFRT>CsChrimson:mVenus	Takayanagi-Kiya and Kiya77	N/A
w;; pJFRC-10xUAS-IVS-eGFP-Kir2.1;	Janelia Research Campus	N/A
Software and algorithms
R	Open source	RRID:SCR_001905
natverse (R package)	Open source, Bates et al.78	https://github.com/natverse
MATLAB	MathWorks	RRID:SCR_001622
ScanImage 2020	Vidrio Technologies	RRID:SCR_014307
Python	Open source	RRID:SCR_008394
CATMAID	Open source, Saalfeld et al.79	RRID:SCR_006278
Fiji	Open source	RRID:SCR_002285
Bonsai	Open source	RRID:SCR_017218
DeepLabCut	Open source, Mathis et al.27	https://deeplabcut.github.io/DeepLabCut
FicTrac	Open source, Moore et al.80	https://github.com/rjdmoore/fictrac
Deposited data
Custom code for connectomics analyses	this study	https://github.com/jefferislab/2023_cVA_Taisz_Galili https://doi.org/10.5281/zenodo.7853021
Image stack from EM neurons for MIP search	this study	https://github.com/jefferislab/skeleton-to-MIP https://doi.org/10.5281/zenodo.78530219