FIG. 7.

Methylation interference analysis of the EBNA2RE 3′ region. The radioactively labelled and randomly methylated oligonucleotide O40 consisting of positions −217 to −178 of the 3′ region was incubated with HeLa nuclear extracts. The protected guanines are shown for complexes A, B, and C in comparison with the free probe. As a control reaction, a Maxam-Gilbert G reaction is shown on the lefthand side. On the righthand side the sequence of the 3′ region from positions −217 to −178 is shown. Protected sequences are marked with asterisks. Minimal extensions of the protection are indicated by boxes.