Fig. 7.

Following GlcN treatment viability of islet cells was not altered. Murine islets were isolated as outlined in Materials and methods. They were treated with 5 mM GlcN beginning 24 h after isolation, for 72 h. Islets were observed and photographed (upper panels) by three investigators, without knowledge of the experimental conditions. After a gentle centrifugation were subjected to MTT assay. The treatment did not decrease cell viability (lower panel) (while decreased islet aggregation, upper panels). The graph represents the media ± SD of three independent experiments. The difference between treated and control cells was evaluated using Student’s t test. p < 0.05 was considered significant. n = 3. There was a very slight difference between control and treated islets. p = 0.13