Fig. 1.

Transformation of soybean cv. JS335 using Agrobacterium tumefaciens strain EHA105 harboring pCAMBIA1301 and regeneration from modified half-seed explants. a Soybean seeds of cv. JS335; b one-day-old imbibed seeds; c half- seed explants prepared from the imbibed seeds (red arrow indicates the presence of plumule and blue arrow indicates the removal of radicle); d, e primary shoot regenerated on MS medium containing 2.2 µM BAP and 3 mg l⁻¹ hygromycin B after 15 days and 30 days of culture respectively; f, g in vitro rooting of primary shoots on MS medium containing 4.9 µM IBA and 2 mg l⁻¹ hygromycin B after 15 days and 30 days of culture respectively; h putatively transformed soybean plantlet; i transformed soybean plant grown in containment room