Fig. 4.

GUS analysis and molecular confirmation of putative transformants regenerated from modified half-seed explants infected with Agrobacterium tumefaciens strain EHA105 harboring pCAMBIA1301. a Expression of gus in mature leaf from putatively transformed plants; b mature leaf from non-transformed plant; c expression of gus in trifoliate leaves from putatively transformed plants; d trifoliate leaves from non-transformed plant; e expression of gus in flower from putatively transformed plants; f flower from non-transformed plant; g expression of gus in pod from putatively transformed plants; h pod from non-transformed plant; i molecular confirmation for the presence of hpt II in putatively transformed soybean plants. Lane 1: DNA ladder (1 Kb); lane 2: pCAMBIA1301 plasmid (positive control); lane 3: soybean genomic DNA from non-transformed plants (negative control); lanes 4–8: genomic DNA from putatively transformed soybean plants with expected amplicon (500 bp) of hpt II