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. 2023 Aug 4;13:12684. doi: 10.1038/s41598-023-39205-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Size dependant payload delivery. (A) Confocal images of GUVs exposed to phytantriol-based cubosomes containing calcein (40 mM) of 310 nm (left column) and 130 nm (right column). All scale bars shown correspond to 10 micron. GUVs were on average between 8 and 20 micron in diameter. (B) Graphical illustration of the experiment. Structures are not to scale. (C) Distribution of intensity differences for calcein, measured as inner minus outer intensity. 64 GUVs measured in total, across three separate experiments. (D) Intensity profiles across two GUVs (both approximately 15 micron in diameter). Values used for the histogram in C were derived from profiles like these.