Fig. 2. Systemic immunity induced by locally transforming different forms of PR1 to pr1.

a The disease symptom of the Pst DC3000-infected systemic leaves of wild-type (WT) and pr1 mutant pre-infiltrated local leaves with different agents. Agents including the Agrobacterium infiltration buffer (Mock), or Agrobacterium carrying P19 plasmid (P19), buffer with AtCAPE9 (AtCAPE9), or Agrobacterium carrying P19 plasmid together with Agrobacterium carrying native PR1 (P19 + PR1), alanine-substituted PR1 (P19 + PR1^D150A), or CAPE9-truncated PR1 (P19 + PR1^ΔCAPE9) driven by 35S promotor. The local pre-infiltrations were performed on leaves # 7–11, and other leaves without infiltration were used as systemic leaves. After 4 days of local infiltration, both local and systemic leaves were inoculated with Pst DC3000 for 7 days, and collected systemic leaves were for disease symptom assessment. b Levels of disease severity were measured by the percentage of yellow area in the total area for the corresponding photographs using the PIDIQ software⁵². c Log colony-forming units (Log CFU) of Pst DC3000 were measured after 7 days of inoculation. b, c Values are means ± SD of four biological replicates. Each replicate was obtained from the systemic leaves of three individual plants. P values were calculated by one-tailed unpaired t-test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).