Fig. 1. Characterization and gene identification of mhz9.

a Ethylene-response phenotype of mhz9. Seedlings were grown in the dark for 3 d in the absence (air) or presence of 10 μL/L of ethylene (left). Ethylene dose-response curves for root length and coleoptile length of WT (Nipponbare) and mhz9 (right). b Expression levels of ethylene-responsive genes in WT and mhz9 with or without ethylene. Total RNAs were isolated from two-day-old etiolated seedlings treated with or without 10 μL/L of ethylene for 8 h. The relative RNA levels of ethylene-responsive genes were detected by RT-qPCR. OsActin was used for normalization. c Schematic diagrams of MHZ9 (LOC_Os01g69990) gene structure and MHZ9 domain. d Phenotype of MHZ9-overexpressing transgenic lines. a, d Lengths are means ± SD (n > 30 biologically independent samples). The scale bar indicates 10 mm. b, d The asterisks indicate significant differences compared with the corresponding WT controls (**P < 0.01; two-tailed Student’s t-test). The experiments were repeated at least three times with similar results. And one representative set of results was shown. Source data are provided as a Source Data file.