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. 2023 Aug 4;8:109. doi: 10.1038/s41541-023-00706-x

Fig. 3. Stronger antigen-specific CD8+ T cell immune responses induced by the combined immunization of Ad-CAIX/Ad-PD-L1.

Fig. 3

a, b The proliferation of lymphocytes isolated from the spleens of mice in each group after continuous stimulation of CAIX protein (10 μg/ml) was detected by the EdU assay. c Detection of T lymphocytes secreting IFN-γ by ELISPOT assay. d After stimulation with CAIX protein, the proportions of IL-2+CD8+ T cells, IFN-γ+CD8+ T cells, and TNF-α+CD8+ T cells in splenocytes from each group were detected by flow cytometry. eg Statistical analysis of the percentages of IL-2+CD8+ T cells, TNF-α+CD8+ T cells, and IFN-γ+CD8+ T cells in d. Data presented as mean ± SD from one representative experiment of three performed. The different significance was set at ***p < 0.001 and ****p < 0.0001. Multiple groups of comparison data were analyzed by one-way ANOVA.