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. 2023 Aug 4;14:4677. doi: 10.1038/s41467-023-39571-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Flow cytometry validation of isolated mouse MDSC (CD11b⁺ Gr-1⁺) and human MDSC (CD11b⁺CD33⁺) from the spleens of tumor-implanted syngeneic mice and PBMC humanized mice, respectively. b Conditioned medium from sgControl cells induced MDSC migration, which was impaired by SLC25A22 knockout (n = 4). Each dot represents an independent sample. c siCXCL1, but not siCXCL3, abrogated induction of MDSC migration in sgControl cell conditioned medium (n = 4). Each dot represents an independent sample. d Anti-Cxcl1 neutralizing antibody (0.25 µg/mL) suppressed MDSC migration in sgControl cell conditioned medium; but had no effect on SLC25A22 knockout cells (CT26: n = 3; Colo26: n = 4). Each dot represents an independent sample. e Recombinant Cxcl1 (1 ng/ml) rescued MDSC migration in CT26-Slc-KO and Colo26-Slc-KO conditioned medium (n = 4). Each dot represents an independent sample. f CXCR2 inhibitors SX682 (2 μM) or SB265610 (10 μM) abolished MDSC migration in sgControl cell conditioned medium (n = 4). Each dot represents an independent sample. g Tumoral CXCL1 positively correlated with MDSC infiltration in Apc^Min/+Kras^G12D/+ organoids (n = 10) in C57BL/6 mice, CT26 allografts (n = 9) in BALB/c mice, and DLD1 xenografts (sgControl, n = 15; SLC-KO1, n = 10) in PBMC humanized mice. Each dot represents an independent mouse. h qPCR of isolated MDSC from CT26 allografts showed that activation markers PD-L1, iNOS, and ARG1 were enhanced in intratumoral MDSC compared to that of splenic MDSC, and was abrogated in SLC25A22 knockout tumors (n = 3). Each dot represents an independent mouse. i Flow cytometry of MDSC from CT26 allografts mice showed that surface PD-L1 protein on MDSC were induced in tumors compared with spleen, which was impaired in SLC25A22 knockout tumors (spleen: n = 3; tumors: n = 10). Each dot represents an independent sample. j SB265610 suppressed the growth of CT26-sgControl allografts (n = 10) and k downregulated MDSC (n = 9), but had no effect on CT26-Slc-KO allografts in BALB/c mice. MDSC positively correlated with tumor weight (n = 36). Each dot represents an independent tumor. Data are shown as mean ± SD. Two-tailed one-way ANOVA (b—d, f, h–k). Two-tailed Student’s t test analysis for two-group comparison e. Spearman correlation test (g, k). Source data are provided as a Source Data file.