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. 2023 Aug 4;13:12666. doi: 10.1038/s41598-023-39643-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Western blots of the hypothalami whole cell lysates determined changes in synaptic proteins in Fmr1 KO male mice. Hypothalami were dissected and protein levels were analyzed by western blotting. Protein levels of 4–8 mice per group were quantified using ChemiDoc and amount of the protein of interest normalized to the amount of β-tubulin. Each point represents one animal, while bars represent group means ± standard error. Statistical significance, indicated with a * (p < 0.05) was determined with t-test followed by Tukey’s post hoc test.